Genomics Data 10 (2016) 151–152 Contents lists available at ScienceDirect Genomics Data journal homepage: www.elsevier.com/locate/gdata Data in Brief Draft whole-genome sequence of the Diaporthe helianthi 7/96 strain, causal agent of sunflower stem canker Riccardo Baroncelli a, Felice Scala b,c, Mariarosaira Vergara d, Michael R. Thon e, Michelina Ruocco c,⁎ a Universitaire de Biodiversité et Ecologie Microbienne (LUBEM), University of Western Brittany, Avenue du Technopole, 29280 Plouzané, France Dipartimento di Agraria, via Università 100, 80055 portici (NA), Italy Istituto per la Protezione Sostenibile delle Piante, sede secondaria di Portici, (IPSP-CNR) via Università 133, 80055 Portici (NA), Italy d Scuola Normale Superiore di Pisa, piazza dei Cavalieri 7, 56126 Pisa, Italy e Instituto Hispano-Luso de Investigaciones Agrarias (CIALE), University of Salamanca, Campus de Villamayor, C/ Del Duero, 12; 37185 Villamayor (Salamanca), Spain b c a r t i c l e i n f o Article history: Received 12 October 2016 Received in revised form 3 November 2016 Accepted 9 November 2016 Available online 11 November 2016 a b s t r a c t Diaporthe helianthi is a fungus pathogenic to sunflower. Virulent strains of this fungus cause stem canker with important yield losses and reduction of oil content. Here we present the first draft whole-genome sequence of the highly virulent isolate D. helianthi strain 7/96, thus providing a useful platform for future research on stem canker of sunflower and fungal genomics. The genome sequence of the D. helianthi isolate 7/96 was deposited at DDBJ/ ENA/GenBank under the accession number MAVT00000000 (BioProject PRJNA327798). © 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Specifications [standardized info for the reader] Organism/cell line/tissue Sex Sequencer or array type Data format Experimental factors Experimental features Consent Sample source location Diaporthe helianthi strain 7/96 N/A Illumina HiSeq 2000 Assembled Fungal strain pathogen on sunflower De novo genome sequencing followed by assembly and annotation N/A D. helianthi 7/96 was isolated from diseased sunflowers in France 1. Direct link to deposited data https://www.ncbi.nlm.nih.gov/bioproject/327798 2. Experimental design, materials and methods Diaporthe helianthi Munt.-Cvet [1], anamorph Phomopsis helianthi, is one of the causal agent of stem canker of sunflower (Helianthus ⁎ Corresponding author. E-mail address: michelina.ruocco@ipsp.cnr.it (M. Ruocco). annuus). The fungus belongs to division Ascomycota, subdivision Pezizomycotina, class Sordariomycetes, order Diaporthales, family Diaporthaceae. Disease develops on leaves by causing brown necrotic lesions at the edge of the leaf blade and then spreads through the veins to the petiole. The leaf dries and remains attached to the stem. From the junction of the leaf the pathogen gradually infects the stem causing diffuse necrosis. The main loss is the shriveling of capitula and lodging of stem, which breaks at different levels. The fungus overwinters as mycelium and perithecia on stem debris left on the soil. Ascospores are released as a yellowish jelly soluble in rainwater. The first contamination of the leaves by ascospores can occur in early spring. D. helianthi 7/96 was isolated from diseased sunflowers in France. This isolate belongs to a group of highly virulent isolates characterized by the presence of a gene fragment having homologies with Aspergillus terreus lovastatin nonaketide polyketide synthase gene (lovB) [2]. The genome of D. helianthi strain 7/96 was sequenced using Illumina mate-paired sequencing technology by BGI HONG KONG CO (BGIHONGKONG CO., Limited, 16 Dai Fu Street, Tai Po Iindustrial Estate, Tai Po, New Territories, Hong Kong). Mate-paired reads of 90 bp (1.80 Gbp; average coverage 38.80×) were assembled using Velvet V 1.2.10 [3]. The genome of D. helianthi consists of 13,383 sequence scaffolds with a total assembly length 46.50 Mbp (N50 = 6347 and N90 = 1502), 51.10% GC-content, and a maximum scaffold size of 54,299 bp. The completeness of the assembly was assessed using BUSCO v12 [4], which estimated the genome sequence to be 98.26% http://dx.doi.org/10.1016/j.gdata.2016.11.005 2213-5960/© 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). 152 R. Baroncelli et al. / Genomics Data 10 (2016) 151–152 complete. The genome was annotated using the MAKER2 pipeline [5]. Overall, 14,220 protein-coding gene models were predicted. Analysis with SignalP 4.1 [6] revealed that 1433 predicted proteins (9.92% of the proteome) contain a secretion signal peptide. Among those 45 are species-specific as they do not have any sequence similarity to proteins in public databases, based on BLAST searches (e-value threshold of 1e-5). Such features are characteristic of fungal effectors, which are proteins that have key roles in restricting the host defense [7]. A first comparative analysis within Diaporthales [8] and model organisms with publically available genomes [Fusarium [9], Neurospora [10], Colletotrichum [11], Magnaporthe [12]] evidenced that D. helianthi 7/96 genome contains a remarkable number (at least 40) of putative polyketide synthases (PKSs). Analysis of PKS gene family in strain 7/96 could be an intriguing starting point to unravel D. helianthi sunflower interactions. 2.1. Nucleotide sequence accession numbers This Whole-Genome Shotgun project has been deposited in GenBank under the accession N°: MAVT00000000 (BioProject PRJNA327798). The version described in this paper is: MAVT00000000.1. Funding information This work was supported by a dedicated grant from the Italian Ministry of Economy and Finance to the National Research Council for the project Innovazione e Sviluppo del Mezzogiorno - Conoscenze Integrate per Sostenibilità ed Innovazione del Made in Italy Agroalimentare - Legge n. 191/2009. References [1] M. 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