ISTA Comparative Test for Forest Tree Seeds

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1 ISTA Comparative Test for Forest Tree Seeds
Elena Foffová Forest Research Institute Zvolen Research Station Liptovský Hrádok Vladimír Foff Forestry Information Agency Liptovský Hrádok

2 ISTA Laboratories CZDL02 - Výzkumní ústav lesního hospodářství a myslivosti, Výzkumná stanice Uherské Hradiště DEDL04 - Staatliche Landwirtschaftliche Untersuchungs- und Forschungsanstalt Augustenbers, Karlsruhe FRDL01 - Centre de Nancy, Unité Recherches Semences Forestiers, Champenoux FRDL02 - Station Nationale d´Essais de Semences, Beaucouzé GBDL02 - Official Seed Testing Station for Tree and Shrubs Seed, Alice Holt Lodge, Wrecclesham, Farnham, Surrey JPDL04 - Forestry and Forest Product Research Institute, Ibaraki NODL02 - Norwegian Forest Seed Station, Hamar RODL02 - Institutul de Cercetari si Amenajari Silvice, Laboratorul de Seminte Foresti, Bucuresti SEDL05 - Seed Laboratory, Dept. of Silviculture, The Swedish University of Agr. Science, Umea SEDL06 - Skog Forsk, The Forestry Research Institute of Sweden, Sävar SIDL01 - Kmetijski institut Slovenije, Seed Testing Labolatory, Ljubljana SKDL02 - Lesnícky výskumný ústav, Výskumná stanica Liptovský Hrádok USDL03 - NationalNational Tree Seed Laboratory, Dry Branch, Georgia

3 Participating Laboratories
Bayerische Landesanstalt für Forstliche Saat- und Pflanzenzucht, Teisendorf Danisch Tree Improvement Station, Humlebak National Forest Genetic Resources Centre, Natural Resource Canada, Canadina Forest Servis - Atlantic, Fredericton, New Brunswick Forstliche Bundesversuchsanstalt, Abteilung für forstliches Vermehrungsgut, Wien Instytut badawczy leśnictwa, Warszawa Landesforstanstalt Eberswalde, Forstsaatgutprüfstelle Waldsieversdorf

4 Obligatory Samples and Tests Facultative 1 Larix decidua Purity Weight
Germination 2 Picea abies 3 4 Pinus sylvestris 5 Facultative 6 Fagus sylvatica Tetrazolium 7

5 Purity analysis Difficulties:
carried out on one whole sample by 14 laboratories on two subsamples by 5 laboratories Difficulties: Results no based on sum of weights of components (3 laborotories) Pure seed definitions No. 47 and 51: distinguishing between winged seeds and seeds without wing (3 laboratories - no, other - big variability) Counting mistakes, rounding

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8 Weight determination Difficulties:
Counting the working sample - 5 laboratories Counting replicates - 14 laboratories Difficulties: One completelly wrong result, weight not calculated from replicates, coefficient of variation not given or not correct calculated, rounding

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11 Germination (conifers)
Quite uniform results in category of normal seedlings at the end of the test but very inconsistent in first counting (after 7 days) Big differences in categories of the ungerminated seeds (hard, fresh, dead, empty) Difficulties: wrong counting or rounding, results not rounded

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16 Facultative tetrazolium and germination tests with beech seeds
High variability in results among laboratories, among the four replicates of the test Average germination (No6 = 37%, No7 = 28%) much lower than the average viability in the TZ test (No6 = 58%, No7 = 57%) Difficulties: not correct counting of results, rounding, exceeded tolerances among replicates, not finished test

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19 Conclusions for next FTS Committee activities:
Purity: to revise PSD of conifer seeds Germination: to revise the definition of „normal seedling“ TZ and germination test of dormant seeds: discuss in detail testing and evaluation procedures (substrates, evaluation of normal seedlings) Results support the FTS Committee goals: to revise the Tree and shrub seed handbook to organise this workshop with practical training