Characterization and fitness of Fusarium graminearum mutants resistant to fluazinam,Plant Pathology

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Characterization and fitness of Fusarium graminearum mutants resistant to fluazinam
Plant Pathology ( IF 2.7 ) Pub Date : 2023-05-06 , DOI: 10.1111/ppa.13739
Dongya Shi ₁ , Xin Li ₁ , Yingying Cao ₁ , Qiankun Li ₁ , Jin Wang ₁ , Zhihui Zhang ₁ , Jane Ifunanya Mbadianya ₁ , Changjun Chen ₁

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Fusarium head blight (FHB), predominately caused by ascomycete Fusarium graminearum sensu stricto, is a devastating fungal disease of cereal crops worldwide. In this study, baseline sensitivity of F. graminearum s. str. to fluazinam was established using 100 isolates that were collected from Anhui and Jiangsu provinces of China in 2009 and 2018. The EC₅₀ values of fluazinam on mycelial growth inhibition ranged from 0.0211 to 0.1097 μg/mL and the mean EC₅₀ was 0.0601 ± 0.0191 μg/mL, which indicated that fluazinam had a strong inhibitory effect on mycelial growth of F. graminearum s. str. To acquire fluazinam-resistant (Flu^R) mutants, one wild-type strain (PH-1, with assembled genome) and another four fluazinam-sensitive (Flu^S) isolates randomly selected from the above-mentioned population were domesticated with fluazinam or mutagenized by ultraviolet light in vitro. As a result, 45 Flu^R mutants were obtained with resistance frequencies of 0.68% (domestication) and 8 in 10⁻⁸ (mutagenesis), respectively. Of the 45 Flu^R mutants, 11 were selected to determine the biological fitness. Based on data of radial mycelial growth, asexual and sexual reproduction, response to environmental stresses, pathogenicity and deoxynivalenol production, all the tested mutants suffered from a fitness penalty. Additionally, slightly positive cross-resistance was observed between the combination of fluazinam and tebuconazole, pyraclostrobin, or pydiflumetofen, but not phenamacril or carbendazim. Therefore, a low-to-moderate risk of development of F. graminearum s. str. resistant to fluazinam was suggested, indicating that fluazinam could be a good alternative fungicide against the FHB in the field.

更新日期：2023-05-06

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