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Evaluation of the mycological status of luncheon meat with special reference to aflatoxigenic moulds and aflatoxin residues

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Abstract

The luncheon meat samples analyzed, which were produced locally by the two main luncheon meat producing companies in Egypt were relatively highly contaminated either by moulds and yeasts in general, aflatoxigenic species and aflatoxin residues in particular. The most frequently encountered fungi from the samples were yeasts, Aspergillus niger, A. flavus, Penicillium chrysogenum, Rhizopus stolonifer, Mucor circinelloides. Less common were Cladosporium sphaerospermum, Alternaria alternata, Mycosphaerella tassiana, P. aurantiogriseum and P. oxalicum. The most important aflatoxigenic species, A. flavus, was isolated frequently. It was 10% of the total fungal isolates from both samples of the two companies. Seven luncheon meat samples out of 50 analyzed were positive for aflatoxin B1 or B1 and G1, while all samples were negative for aflatoxins B2, G2, M1 and M2. Aflatoxin B1 was detected only in 4 and 3 samples out of 25 analyzed from each of company A and B, respectively. The highest detectable level, 11.1 ppb, was recorded in a sample from company B and the least, 0.5 ppb, in a sample from company A. Aflatoxin G1, at concentration of 3.2 ppb, was detected in only one sample of the aflatoxin B1 – contaminated 3 samples of company B: this sample also had the highest level of aflatoxin B1. Some luncheon meat samples had higher numbers of aflatoxigenic A. flavus than others, however these samples were negative for aflatoxins. The hazardous potential of such contamination will be discussed.

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References

  1. Mossel DAA. Microbiology of food. 3rd ed. The University of Ulterecht, The Netherlands, 1982.

    Google Scholar 

  2. Diener UL, Davies ND, Salmon WD, Prickett CO. Toxinproducing Aspergillus isolated from domestic peanuts. Science 1963; 142: 1491–1492.

    PubMed  CAS  Google Scholar 

  3. Wogan GN, Paglialunga S, Newberue PN. Carcinogenic effect of low dietary levels of aflatoxin B1 in rats. Food Cosmet Toxicol 1974; 12: 681–685.

    Article  PubMed  CAS  Google Scholar 

  4. Chu FS. Mode of action of mycotoxins and related compounds. Adv Appl Microbiol 1977; 40: 352–357.

    Google Scholar 

  5. Hsieh DPH. The role of aflatoxins in human cancer. In: Steyn PS, Viggar R, eds. Mycotoxins and phytotoxins. Amsterdam: Elsevier Science 1986: 447–456.

    Google Scholar 

  6. Pitt JI, Hocking AD. Fungi and food spoilage. 2nd ed. Blackie Academic & Professional, London, U.K., 1997, 593 pp.

    Google Scholar 

  7. Ranken MD. Meat and meat products. In: Food Industries Manual, 21st ed. Chapter 1, 1984, 18 pp, Leonard Hill, USA.

    Google Scholar 

  8. King AD, Hocking AD, Pitt JI. Dichloran-rose bengal medium for enumeration and isolation of molds from foods. Appl Environ Microbiol 1979; 37: 959–964.

    PubMed  Google Scholar 

  9. Raper KB, Fennell DI. The genus Aspergillus. Baltimore, Williams and Wilkins, 1965, 686 pp.

    Google Scholar 

  10. Ellis MB. Dematiaceous Hyphomycetes. Kew Surrey: Commonwealth Mycological Institute 1971: 608 pp.

    Google Scholar 

  11. Ellis MB. More Dematiaceous Hyphomycetes. Kew, Surrey: Commonwealth Mycological Institute 1976: 507 pp.

    Google Scholar 

  12. Booth C. Fusarium: Laboratory Guide to the Identification of the Major Species. Kew, Surrey: Commonwealth Mycological Institute 1977; 58 pp.

    Google Scholar 

  13. Pitt JI. The genus Penicillium and its Teleomorphic States Eupenicillium and Talaromyces. London: Academic Press, 1979, 634 pp.

    Google Scholar 

  14. Domsch KH, Gams W, Anderson T-H. Compendium of Soil Fungi. London: Academic Press 1980; 859 pp.

    Google Scholar 

  15. Sivanesan A. Graminicolous species of Bipolaris, Curvularia, Drechslera, Exserohilum and their teleomorphs. Mycological Papers 1987; 158: 1–261, C.A.B. International Mycological Institute, Ferry Lane, Kew, Surrey, UK

    Google Scholar 

  16. Samson RA, van Reenen-Hoekstra ES. Introduction to foodborne fungi. Centraalbureau voor Schimmelcultures, Baarn, The Netherlands, 1988, 299 pp.

    Google Scholar 

  17. Kozakiewicz Z. Aspergillus Species on Stored Products. Mycological Papers 1989; 161: 1–188, C.A.B. International Mycological Institute, Ferry Lane, Kew, Surrey, UK.

    Google Scholar 

  18. Moubasher AH. Soil fungi in Qater and other Arab countries. The Scientific and Applied Research Centre, University of Qater, Doha, Qatar, 1993, 566 pp.

    Google Scholar 

  19. Association of Official Analytical Chemists. Official Methods of Analysis. 13th ed, Benjamin, Franklin stat, Washington, DC 1980.

    Google Scholar 

  20. Kalton G. Introduction to statistical ideas from social scientist. 2nd ed. London: Academic Press, 1967.

    Google Scholar 

  21. Abdel-Rahman HA, Yossef H, Hefnawey Y. Mycological quality of meat products in Egypt. Assiut Vet Med J 1984; 12(24): 153–159.

    Google Scholar 

  22. Roushdy S, Ibrahim A, Aldanaf N, Hammad H, Moustafa R. Mycotoxin residues in meat and meat products. Vet Med J, Giza 1996; 44(2): 181–187.

    CAS  Google Scholar 

  23. Lotfi A, Abd El-Rahman HA, Hefnawy Y, Youssef H. Studies on the mycological status of sausage in Upper Egypt. Fleischwirtschaft 1983; 63(4): 595–596.

    Google Scholar 

  24. Grazia L, Romano P, Bagni A, Roggiani D, Guglielmi G. The role of moulds in the ripening process of salami. Food Microbiol 1986; 3: 19–25.

    Article  Google Scholar 

  25. Mutti P, Previdi MP, Quintavalla S, Spotti E. Toxigenicity of mould strains isolated from salami as a function of culture medium. Ind Conserve 1988; 63: 142–145.

    Google Scholar 

  26. Kaur H, Joshi DV, Kwatra MS, Jand SK. Preliminary studies on mycoflora of pig sausages. Ind J Comp Microbiol Immunol Infect Dis 1992; 13: 31–33.

    Google Scholar 

  27. Andersen SJ. Compositional changes in surface mycoflora during ripening of naturally fermented sausages. J Food Prot 1995; 58: 426–429.

    Google Scholar 

  28. Abdel-Rahman H, Darwish A, Hamdy M. Mould affections of a meat cold store. Assiut Vet Med J 1985; 14(27): 131–134.

    Google Scholar 

  29. Mansour NK, Yassien NA, Darwish AM, EI-Sherief AM. Psychrotrophic spoilage moulds in imported frozen beef cuts. Vet Med J, Giza 1991; 39: 209–219.

    Google Scholar 

  30. Nassar A, Ismail MA. Psychrotrophic and mesophilic fungi isolated from imported frozen lean meat in Egypt. J Food Safety 1994; 14: 289–295.

    Google Scholar 

  31. Filtenborg O, Frisvad JC, Thrane U. Moulds in food spoilage. Int J Food Microbiol 1996; 33: 85–102

    Article  PubMed  CAS  Google Scholar 

  32. Inagaki N. On some fungi isolated from foods I. Trans Mycol Soc Japan 1962; 4: 1–5.

    Google Scholar 

  33. Refai M, Loot A. Studies of mould contaminations of meat in slaughter houses, butcher's shops and in cold stores. Mykosen 1969; 12(10): 621–624.

    PubMed  CAS  Google Scholar 

  34. Hadlok R, Schipper MAA. Schimmelpilze und Fleisch: Reihe Mucorales. Fleischwirtschaft 1974; 54: 1796–1800.

    Google Scholar 

  35. Aziz NH, Youssef YA. Occurrence of aflatoxins and aflatoxin producing moulds in fresh and processed meat in Egypt. Food Addit Contam 1991; 8(3): 321–331.

    PubMed  CAS  Google Scholar 

  36. Ismail MA, Abou Elala A-H, Nassar A, Michail DG. Fungal contamination of beef carcasses and the environment in a slaughterhouse. Food Microbiol 1995; 12: 441–445.

    Article  Google Scholar 

  37. Yassein N, Mansour N, Eldaly E, Darwish A. Contamination of slaughtered camels, cattle and their surroundings with moulds in urban abattoir. Alex J Vet Sci 1989; 5(2): 185–193.

    Google Scholar 

  38. Mansour N, Hamdy M, Yassien N, Refai M. Dematiaceous hyphomycetes in slaughtered camels, cattle and surroundings at Cairo abattoir. Assiut Vet Med J 1990; 24(47): 145–152.

    Google Scholar 

  39. Nassar A, Ismail MA, Abou Elala A-H. The role of benzoic acid as a fungal decontaminant of beef carcases. Fleischwirtschaft 1995; 75(10): 1218–1220.

    CAS  Google Scholar 

  40. Kurtzman CP, Horn BW, Hesseltine CW. Aspergillus nomius, a new aflatoxin-producing species related to Aspergillus flavus and Aspergillus tamarii. Antonie van Leeuwenhoek 1986; 53: 147–158.

    Article  Google Scholar 

  41. Wilson DM, Payne GA. Factors affecting A. flavus group infection and aflatoxin contamination of crops. Academic Press, New York, 1994.

    Google Scholar 

  42. Bullerman LB. Mycotoxins and food safety. Food Technol 1986; 40: 59–66.

    Google Scholar 

  43. Zohri AA, Abdel-Sater MA, Ismail MA. Incidence of aflatoxins and mould flora in corn snacks. J Food Sci Technol 1995; 32(4): 289–294.

    CAS  Google Scholar 

  44. Roegner FR. FAD Looks at aflatoxin. 38th Ann Technol Conf, Grain Elevtors Proc Supl Assn, Chicago, 1967.

    Google Scholar 

  45. Allcroft R, Carnagham RBA. Groundnut toxicity: an examination for toxin in human food products from animals fed toxic groundnut meal. Vet Rec 1963; 75: 259–263.

    CAS  Google Scholar 

  46. Keyl AC, Booth AN, Nasri MS, Gumbmann MR, Gagne WE. Chronic effects of aflatoxin in farm animal feeding studies. In: Harzberg M, ed. Proceedings 1st U.S. Japan Conf. on Toxic Microorganisms, Honolulu. UNJNR joint panels and U.S. Department of interior, Washington, DC 1970, pp. 72–75.

    Google Scholar 

  47. Purchase IFH. The control of aflatoxin residues in food of animal origin. Pure Appl Chem 1973; 35: 283–288.

    CAS  Google Scholar 

  48. Feng YC, Tang JR. Survey of aflatoxin contamination of feeds in Guangxi zhuang Autonomous Region, China. Chin J Vet Med 1990; 16(6): 16–18.

    Google Scholar 

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Authors and Affiliations

  1. Department of Botany, Faculty of Science, Assiut University, Assiut, Egypt

    M. A. Ismail

  2. Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt

    Z. M. Zaky

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  1. M. A. Ismail
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  2. Z. M. Zaky
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Ismail, M.A., Zaky, Z.M. Evaluation of the mycological status of luncheon meat with special reference to aflatoxigenic moulds and aflatoxin residues. Mycopathologia 146, 147–154 (1999). https://doi.org/10.1023/A:1007086930216

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