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Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet’s daffodil (Narcissus poeticus L.) in Serbia

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Abstract

In autumn 2018, daffodil (narcissus) plants with basal bulb rot were noticed in a plantation in locality Jasika, Serbia. Morphological characterization identified Fusarium oxysporum f. sp. narcissi as the causal agent of bulb rot in daffodil. Three selected isolates were tested for their ability to produce symptoms on daffodil bulbs. Two weeks after artificial inoculation typical symptoms of basal rot developed on inoculated bulbs from which the pathogen was re-isolated fulfilling Koch’s postulates. To further identify the pathogen, a molecular characterization with sequence and phylogenetic analysis of ITS and TEF1 genomic regions was performed. Molecular characterization confirmed the obtained results in morphological identification. The detection of Fusarium oxysporum f. sp. narcissi, a causal agent of basal rot on daffodil in Serbia, is the first report of this pathogen in the country.

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References

  • Burgess LW, Summerell BA, Bullock S, Gott KP, Backhouse D (1994) Laboratory manual for Fusarium research, 3rd edn. Fusarium Research Laboratory, Department of Crop Sciences, University of Sydney and Royal Botanic Gardens, Sydney

  • Day JP, Shattock RC (1997) Aggressiveness and other factors relating to displacement of populations of Phytophthora infestans in England and Wales. Eur J Plant Pathol 103:379–391. https://doi.org/10.1023/A:1008630522139

    Article  Google Scholar 

  • Edel-Hermann V, Lecomte C (2019) Current status of Fusarium oxysporum formae speciales and races. Phytopathology 109:512–530. https://doi.org/10.1094/PHYTO-08-18-0320-RVW

    Article  PubMed  CAS  Google Scholar 

  • Glass NL, Donaldson GC (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 61:1323–1330

    Article  CAS  Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acid S 41:95–98

    CAS  Google Scholar 

  • Hanks GR (2013) Narcissus manual. Kenilworth, AHDB Horticulture, Warwickshire, UK

  • Hanks GR (1996) Control of Fusarium oxysporum f. sp. narcissi, the cause of narcissus basal rot, with thiabendazole and other fungicides. Crop Prot 15:549–558. https://doi.org/10.1016/0261-2194(96)00023-3

    Article  CAS  Google Scholar 

  • Hanks G.R., Chastagner G.A. (2018) Diseases of Daffodil (Narcissus). In: McGovern R, Elmer W (eds) Handbook of florists’ crops diseases. Handbook of plant disease management. Springer, Cham. https://doi.org/10.1007/978-3-319-39670-5_43

  • Hegde KT, Narayanaswamy H, Veeraghanti KS (2018) Morphological and cultural characteristics of Fusarium oxysporum f. sp. dianthi under laboratory condition. J Pharmacogn Phytochem 7(4):2839–2842

  • Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549. https://doi.org/10.1093/molbev/msy096

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Leslie JF, Summerell BA (2008) The Fusarium Laboratory Manual. Wiley, Hoboken

    Google Scholar 

  • Mohamed RA, Al-Bedak OA, Hassan SHA (2021) First record in Upper Egypt of vascular wilt on pomegranate caused by Fusarium oxysporum, its molecular identification and artificial pathogenicity. J Plant Dis Prot 128:311–316. https://doi.org/10.1007/s41348-020-00385-z

    Article  Google Scholar 

  • Nelson PE, Toussoun TA, Marasas WFO (1983) Fusarium species: an illustrated manual for identification. Pennsylvania State University Press, USA

    Google Scholar 

  • O’Donnell K, Cigelnik E (1997) Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Mol Phylogenet Evol 7:103–116. https://doi.org/10.1006/mpev.1996.0376

    Article  PubMed  CAS  Google Scholar 

  • Taylor A, Armitage DA, Handy C, Jackson CA, Hulin TM, Harrison JR, Clarkson PJ (2019) Basal rot of narcissus: Understanding pathogenicity in Fusarium oxysporum f. sp. narcissi. Front Microbiol 10(2905):1–17. https://doi.org/10.3389/fmicb.2019.02905

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications, Academic Press, New York, USA

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Funding

This work was supported by the Fruit Research Institute, Čačak, Serbia.

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Authors and Affiliations

  1. Faculty of Agriculture, University of Niš, Kosančićeva 4, 37000, Kruševac, Serbia

    Tanja Vasić & Sanja Živković

  2. Fruit Research Institute, Kralja Petra I 9, 32000, Čačak, Serbia

    Darko Jevremović

  3. Institute for Animal Husbandry, Autoput 16, 11080, Belgrade, Serbia

    Vesna Krnjaja

  4. Agricultural Institute of Republic of Srpska, Knjaza Miloša 17, Banja Luka, Bosnia and Herzegovina

    Vojislav Trkulja

Authors
  1. Tanja Vasić
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  2. Darko Jevremović
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  3. Vesna Krnjaja
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  4. Sanja Živković
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  5. Vojislav Trkulja
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Correspondence to Tanja Vasić.

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Vasić, T., Jevremović, D., Krnjaja, V. et al. Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet’s daffodil (Narcissus poeticus L.) in Serbia. J Plant Dis Prot 128, 1357–1361 (2021). https://doi.org/10.1007/s41348-021-00476-5

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  • DOI: https://doi.org/10.1007/s41348-021-00476-5

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