Abstract
In autumn 2018, daffodil (narcissus) plants with basal bulb rot were noticed in a plantation in locality Jasika, Serbia. Morphological characterization identified Fusarium oxysporum f. sp. narcissi as the causal agent of bulb rot in daffodil. Three selected isolates were tested for their ability to produce symptoms on daffodil bulbs. Two weeks after artificial inoculation typical symptoms of basal rot developed on inoculated bulbs from which the pathogen was re-isolated fulfilling Koch’s postulates. To further identify the pathogen, a molecular characterization with sequence and phylogenetic analysis of ITS and TEF1 genomic regions was performed. Molecular characterization confirmed the obtained results in morphological identification. The detection of Fusarium oxysporum f. sp. narcissi, a causal agent of basal rot on daffodil in Serbia, is the first report of this pathogen in the country.
References
Burgess LW, Summerell BA, Bullock S, Gott KP, Backhouse D (1994) Laboratory manual for Fusarium research, 3rd edn. Fusarium Research Laboratory, Department of Crop Sciences, University of Sydney and Royal Botanic Gardens, Sydney
Day JP, Shattock RC (1997) Aggressiveness and other factors relating to displacement of populations of Phytophthora infestans in England and Wales. Eur J Plant Pathol 103:379–391. https://doi.org/10.1023/A:1008630522139
Edel-Hermann V, Lecomte C (2019) Current status of Fusarium oxysporum formae speciales and races. Phytopathology 109:512–530. https://doi.org/10.1094/PHYTO-08-18-0320-RVW
Glass NL, Donaldson GC (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 61:1323–1330
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acid S 41:95–98
Hanks GR (2013) Narcissus manual. Kenilworth, AHDB Horticulture, Warwickshire, UK
Hanks GR (1996) Control of Fusarium oxysporum f. sp. narcissi, the cause of narcissus basal rot, with thiabendazole and other fungicides. Crop Prot 15:549–558. https://doi.org/10.1016/0261-2194(96)00023-3
Hanks G.R., Chastagner G.A. (2018) Diseases of Daffodil (Narcissus). In: McGovern R, Elmer W (eds) Handbook of florists’ crops diseases. Handbook of plant disease management. Springer, Cham. https://doi.org/10.1007/978-3-319-39670-5_43
Hegde KT, Narayanaswamy H, Veeraghanti KS (2018) Morphological and cultural characteristics of Fusarium oxysporum f. sp. dianthi under laboratory condition. J Pharmacogn Phytochem 7(4):2839–2842
Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549. https://doi.org/10.1093/molbev/msy096
Leslie JF, Summerell BA (2008) The Fusarium Laboratory Manual. Wiley, Hoboken
Mohamed RA, Al-Bedak OA, Hassan SHA (2021) First record in Upper Egypt of vascular wilt on pomegranate caused by Fusarium oxysporum, its molecular identification and artificial pathogenicity. J Plant Dis Prot 128:311–316. https://doi.org/10.1007/s41348-020-00385-z
Nelson PE, Toussoun TA, Marasas WFO (1983) Fusarium species: an illustrated manual for identification. Pennsylvania State University Press, USA
O’Donnell K, Cigelnik E (1997) Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Mol Phylogenet Evol 7:103–116. https://doi.org/10.1006/mpev.1996.0376
Taylor A, Armitage DA, Handy C, Jackson CA, Hulin TM, Harrison JR, Clarkson PJ (2019) Basal rot of narcissus: Understanding pathogenicity in Fusarium oxysporum f. sp. narcissi. Front Microbiol 10(2905):1–17. https://doi.org/10.3389/fmicb.2019.02905
White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications, Academic Press, New York, USA
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This work was supported by the Fruit Research Institute, Čačak, Serbia.
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Vasić, T., Jevremović, D., Krnjaja, V. et al. Morphological and molecular identification of Fusarium oxysporum f. sp. narcissi on poet’s daffodil (Narcissus poeticus L.) in Serbia. J Plant Dis Prot 128, 1357–1361 (2021). https://doi.org/10.1007/s41348-021-00476-5
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DOI: https://doi.org/10.1007/s41348-021-00476-5