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Weeds as alternate hosts of Xanthomonas euvesicatoria pv. euvesicatoria and X. campestris pv. campestris in vegetable-growing fields in the state of Pernambuco, Brazil

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Abstract

Plant pathogenic Xanthomonas species attack a wide range of agriculture crops and is capable of surviving on weeds. In this study, Xanthomonas strains obtained from weeds grown withing vegetable crop fields in the state of Pernambuco, Northeast Brazil, were identified. Bacterial colonies resembling Xanthomonas were obtained from Aeollanthus suaveolens (strain CCRMXe03), Amaranthus lividus (CCRMXe01 and CCRMXe02), Sida glomerata (CCRMXe04), and Emilia fosbergii (CCRMXe04). All weeds but E. fosbergii exhibited lesions on the leaves. Phylogenetic analysis of the gyrB and rpoD genes and PCR-specific assays allowed to identify X. euvesicatoria pv. euvesicatoria (CCRMXe01, CCRMXe02, CCRMXe03, and CCRMXe04) and X. campestris pv. campestris (CCRMXcc371). All strains but CCRMXe03 were pathogenic to its host of origin. All X. euvesicatoria pv. euvesicatoria strains were pathogenic to leaves and fruits of tomato and bell pepper. Two strains, CCRMXe03 and CCRMXe04, were capable of inducing soft rot in fruits. Amylolytic activity was found in all strains and two strains (CCRMXe03 and CCRMXe04) degraded pectate. Strain CCRMXcc371 was pathogenic to cabbage, kale, cauliflower, and broccoli. This work provides new knowledge of hosts for two important plant pathogenic bacteria for vegetable crops.

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Acknowledgements

This study was financed in part by The Brazilian National Council for Scientific and Technological Development (CNPq), with a scholarship granted to LVSS, the Coordination for the Improvement of Higher Education Personnel (CAPES) - Finance Code 001 (Proc. No. 23038.003635/2013-60) and by Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE), with a research grant to KCSF (AUXPE 1585/2013).

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Authors and Affiliations

  1. Department of Agronomy, Federal Rural University of Pernambuco, 52171-900, Recife, PE, Brazil

    Leandro V. S. Santos, Edilaine A. Melo, Adriano M. F. Silva, Kátia C. S. Félix, Greecy M. R. Albuquerque & Marco A. S. Gama

  2. Embrapa Hortaliças, 70275-970, Brasília, DF, Brazil

    Alice M. Quezado-Duval

  3. Department of Biology, Federal Rural University of Pernambuco, 52171-900, Recife, PE, Brazil

    Elineide B. Souza

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  1. Leandro V. S. Santos
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  2. Edilaine A. Melo
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  3. Adriano M. F. Silva
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Contributions

LVSS, EAM, KCFS and AMFS planed, designed, executed experimental work, conducted data analyses and wrote the manuscript, AMQD and GMRA, conducted data analyses and wrote the manuscript, and MASG and EBS planed, designed and wrote the manuscript.

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Correspondence to Elineide B. Souza.

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Santos, L.V.S., Melo, E.A., Silva, A.M.F. et al. Weeds as alternate hosts of Xanthomonas euvesicatoria pv. euvesicatoria and X. campestris pv. campestris in vegetable-growing fields in the state of Pernambuco, Brazil. Trop. plant pathol. 45, 484–492 (2020). https://doi.org/10.1007/s40858-020-00350-z

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