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Host range investigation of Phoma koolunga, a causal agent of ascochyta blight of field pea

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Abstract

Phoma koolunga is one of three species that cause ascochyta blight (synonym: blackspot) of field pea (Pisum sativum L.) in Australia. P. koolunga was first described in 2009 in South Australia and has since been reported in Western Australia and Victoria. However, the origin of the pathogen and its host range remain largely unknown. The aim of this study was to examine the host range of P. koolunga and to explore possible origin(s) of P. koolunga as a pathogen of field pea in Australia. Host range experiments were conducted in a growth room using 41 legumes comprising Australian native, naturalised, crop and pasture legumes, and wild type Pisum, Lathyrus and Vicia spp. Two isolates of P. koolunga from field pea in South Australia were compared. Disease incidence and severity were measured as number of leaves and percentage of leaf area diseased every 4 days up to 12 days post-inoculation. All legumes tested, except Cicer arietinum (chickpea), developed leaf lesions and some also had stem lesions. Disease incidence and severity differed significantly among species but not consistently between the P. koolunga isolates. The ability of the isolates to cause lesions on a wide range of legumes in controlled environment conditions suggests the fungus has a broad host range in humid and mild temperature conditions conducive for disease development. These results provide no evidence to suggest P. koolunga could have evolved with any of the 17 Australian native legume species tested and the fungus is, therefore, unlikely to have originated within Australia.

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Acknowledgements

This research was a part of the Master of Philosophy of the first author. The authors thank Mrs. M. Krysinska-Kaczmarek and Mr. J. Stonor, SARDI, for technical advice, and Dr. S. Hughes, SARDI, and Dr. S. Norton, AGG, for providing seeds. The University of Adelaide School of Agriculture, Food and Wine and SARDI provided financial and logistical support.

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Authors and Affiliations

  1. School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, Adelaide, South Australia, 5005, Australia

    E. C. Keirnan, J. A. Davidson & E. S. Scott

  2. South Australian Research and Development Institute (SARDI), Adelaide, South Australia, 5001, Australia

    J. A. Davidson

  3. Rho Environmetrics, PO Box 366, Highgate, South Australia, 5063, Australia

    R. L. Correll

Authors
  1. E. C. Keirnan
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  2. J. A. Davidson
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  3. R. L. Correll
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  4. E. S. Scott
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Correspondence to E. S. Scott.

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Keirnan, E.C., Davidson, J.A., Correll, R.L. et al. Host range investigation of Phoma koolunga, a causal agent of ascochyta blight of field pea. Australasian Plant Pathol. 49, 707–719 (2020). https://doi.org/10.1007/s13313-020-00747-z

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  • DOI: https://doi.org/10.1007/s13313-020-00747-z

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