Abstract
With an annual loss of up to 100%, anthracnose caused by Colletotrichum is one of the most devastating diseases of common beans (Phaseolus vulgaris L.). Due to few distinctive morphological characters, Colletotrichum species are frequently misidentified. In India, several Colletotrichum species have been reported as pathogens of Phaseolus species, but none had previously been validated by means of molecular tools. In this study, we studied Colletotrichum strains from common beans cv. Bhaderwah-Rajmash from the northern Himalayas of India based on both morphological and DNA sequence data of six loci, namely ITS, gapdh, chs-1, his3, act, tub2. The strains were identified as C. lindemuthianum that belongs to the C. orbiculare species complex. Representative C. lindemuthianum strains tested on Phaseolus vulgaris cv. Bhaderwah-Rajmash were pathogenic and exhibited variation in symptomology and disease progression. By identifying the causal agent, we provided substantial information to develop the best control strategies for anthracnose of Phaseolus vulgaris from the northern Himalayas of India.
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Acknowledgements
The corresponding authors gratefully acknowledges the financial support (San No. 09/1238(0001)/2019-EMR-I) received from the Council of Scientific and Industrial Research (CSIR), Govt. of India and the School of Biotechnology, SKUAST-Jammu for the work reported in this paper. Authors also thanks the Director of the MACS Agharkar Research Institute, Pune, India for laboratory support.
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RKS conceived and designed the experiments and wrote the final manuscript. CG performed experiment and wrote the manuscripts. UD also read and edited the manuscript. RKC helped in conceiving the experiment.
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Gupta, C., Salgotra, R.K., Damm, U. et al. Phylogeny and pathogenicity of Colletotrichum lindemuthianum causing anthracnose of Phaseolus vulgaris cv. Bhaderwah-Rajmash from northern Himalayas, India. 3 Biotech 12, 169 (2022). https://doi.org/10.1007/s13205-022-03216-0
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DOI: https://doi.org/10.1007/s13205-022-03216-0